| |
¿¬¼¼ÇÁ·ÎÅ׿ȿ¬±¸¼¾ÅÍ ºÐ¼®¿ä±Ý (2008³â 5¿ùºÎÅÍ Àû¿ë)
¼ºñ½ºÇ׸ñ |
»ç¿ë Àåºñ |
ÇöÇà
ºÐ¼®¿ä±Ý |
¿ä±Ý Á¶Á¤¾È |
ºñ°í |
¼ºñ½º ³»¿ë |
2-D PAGE
Coomassie
1Àå |
|
170,000¿ø |
200,000¿ø |
17% Àλó
(2005³â 1¿ù ÀÌÈÄ Ã¹Àλó) |
sample preparation Æ÷ÇÔ
2-DE clean-up kit »ç¿ë
18cm, 9-17% gel
Silver staining ¼±Åà °¡´É
Narrow pH separation °¡´É |
Image Analysis
1 pair
|
|
160,000¿ø |
170,000¿ø |
6% Àλó |
Image Master Platinum 5
spot Á¤¼º Á¤·® ºÐ¼® |
MALDI-TOF
1 »ùÇÃ |
4800 TOF/TOF |
100,000¿ø |
100,000¿ø |
ÇöÇà°ú µ¿ÀÏ |
In-gel digestion,
Poros clean-up Æ÷ÇÔ
DB search Æ÷ÇÔ |
| MALDI-TOF/TOF 1 »ùÇÃ |
4800 TOF/TOF |
200,000¿ø |
200,000¿ø |
ÇöÇà°ú µ¿ÀÏ |
In-gel digestion,
Poros clean-up Æ÷ÇÔ
DB search Æ÷ÇÔ |
| LC-MS/MS 1 »ùÇÃ |
LTQ |
190,000¿ø |
200,000¿ø |
5% Àλó
(2005³â 7¿ù ÀÌÈÄ Ã¹Àλó)
|
In-gel digestion, DB search Æ÷ÇÔ |
| MARC 1ȸ running |
Hu-6 column (Agilent) |
65,000¿ø |
70,000¿ø |
7% Àλó
(2005³â 1¿ù ÀÌÈÄ Ã¹Àλó) |
Low abundant protein
100~120 ug depleted
fraction/ running |
| PF2D 1 »ùÇÃ |
Beckman |
1,400,000¿ø |
1,500,000¿ø |
7% Àλó
(2005³â 1¿ù ÀÌÈÄ Ã¹Àλó) |
2-D column separation |
| FFE |
DB |
½Ã°£´ç 230,000¿ø
5½Ã°£¿¡ 910,000¿ø |
½Ã°£´ç 250,000¿ø
5½Ã°£¿¡ 970,000¿ø |
8% Àλó
(2005³â 1¿ù ÀÌÈÄ Ã¹Àλó) |
50~70 fraction |
| DIGE |
GE Healthcare |
3,000,000¿ø |
3,000,000¿ø |
ÇöÇà°ú µ¿ÀÏ |
24 cm DIGE gel 3Àå,
Coomassie gel 1Àå,
Decyder 6.5 Åë°èºÐ¼® |
| 2-DE/ MALDI-TOF Package |
|
2,200,000¿ø |
2,350,000¿ø |
7% Àλó
(2005³â 1¿ù ÀÌÈÄ Ã¹Àλó, Package Àû¿ë½Ã spot´ç 62,000¿ø¿¡¼ 65,000¿øÀ¸·Î ÀλóµÊ) |
2D Coomassie 2Àå
MALDI-TOF 30 spots
(2-DE ¿Í °°ÀÌ ÀÇ·ÚÇÏ´Â °æ¿ì
TOF spot ´ç 65,000¿ø) |
| 2-DE °³º°±³À° ¼ºñ½º |
|
800,000¿ø |
900,000¿ø (846,000¿ø) |
12% Àλó |
»ùÇÃÁ¦Á¶ºÎÅÍ 2-DE Àü°úÁ¤¿¡ ´ëÇÑ ±³À°.
2-DE 2Àå ºÐ¼®À» ±âº»À¸·Î Çϰí,
Ãß°¡µÇ´Â »ùÇÃÀº °¹¼ö¿¡ µû¶ó
2-DE ºÐ¼®¿ä±Ý (200,000¿ø) Àû¿ë |
2008³â 1¿ùºÎÅÍ Ãß°¡µÈ ºÐ¼® ¼ºñ½º Ç׸ñ
| ¼ºñ½ºÇ׸ñ |
ºÐ¼®¿ä±Ý |
¼ºñ½º ³»¿ë |
Phospho-protein
Fluorescence Staining
1Àå |
18~24 cm 350,000¿ø
7cm 150,000¿ø |
Çü±¤ ¿°»ö°ú Çü±¤ ½ºÄµ ÈÄ¿¡ °°Àº gelÀ» Coomassie staining ½ºÄµ±îÁö ÇØÁÖ´Â ºÐ¼®
ProQ Diamond staining Scanning with Typhoon Coomassie staining Scanning |
Glyco-protein
Fluorescence Staining
1Àå |
18~24 cm 350,000¿ø
7cm 150,000¿ø |
Çü±¤ ¿°»ö°ú Çü±¤ ½ºÄµ ÈÄ¿¡ °°Àº gelÀ» Coomassie staining ½ºÄµ±îÁö ÇØÁÖ´Â ºÐ¼®
ProQ Emerald staining Scanning with Typhoon Coomassie staining Scanning |
| Image Analysis 1 pair |
170,000¿ø |
Melanie 7.0 (GeneBio)
spot Á¤¼º Á¤·® ºÐ¼®
Image Master Platinum 5.0 ¿ä±Ý°ú Â÷ÀÌ ¾øÀ½.
|
Phosphopeptides
LC-MS/MS
ºÐ¼® 1 »ùÇà |
200,000¿ø |
Phosphopeptides enrichment kit (PerkinElmer) »ç¿ë
LTQ ºÐ¼®
ÀϹÝÀûÀÎ LC-MS/MS ºÐ¼®°ú ¿ä±ÝÂ÷ÀÌ ¾øÀ½. |
ÁÖ¿ä ºÐ¼® ¼ºñ½º ¼Ò¿ä½Ã°£
2-DE
(18cm, Coomassie, Silver Fluorescent staining) |
7 working day
(1 ~12 sample) |
Sample preparation (Sonication, Ultracentrifuge)
Protein Á¤·® --- 4½Ã°£
Rehydration - 12~18 ½Ã°£
Focusing - 31~35 ½Ã°£
Gel running - 6½Ã°£ ¶Ç´Â 14½Ã°£
Staining -12½Ã°£
Destaining - 7~8½Ã°£
Scanning - 1½Ã°£ |
| Image analysis |
5 working day
( 1 pair) |
|
MALDI-TOF or MALDI TOF/TOF
(ABI 4800 TOF/TOF) |
5 working day (1~30 spots) |
Destaining - 2 ½Ã°£ (per 10 spots)
In-gel digestion - 12 ½Ã°£
Poros desalting, spotting - 2 ½Ã°£
MALDI-TOF - 30ºÐ
Database searching - 4 ½Ã°£ |
Nano-LC-MS/MS
(Finnigan-LTQ ABI Q-STAR) |
5 working day (1~5 sample) |
Destaining - 2 ½Ã°£ (per 10 spots)
In-gel digestion - 12 ½Ã°£
Poros desalting- 2 ½Ã°£
LC-MS - 7½Ã°£
Database searching - 3 ½Ã°£ |
| MARC (Agilent) |
1 working day
(1 running) |
|
| DIGE |
4 weeks |
|
µ¥ÀÌÅÍ ¹ÞÀ¸½Ã´Â °÷ www.webhard.co.kr
ID : webyprc
PW : webyprc
³»¸®±â Àü¿ë Æú´õ
¸ðµç ±â¼ú¼ºñ½º °ü·Ã ¹®ÀÇ´Â º» ¼¾ÅÍÀÇ ÀÌÀº¿µ ¹Ú»ç(02-2123-6625, )¿¡°Ô ¹®ÀÇ ÇϽøé Á»´õ »ó¼¼ÇÑ ¾È³»µé ¹ÞÀ¸½Ç ¼ö
ÀÖ½À´Ï´Ù.
|