Register | Log-in | Sitemap    
   HOME   |   PROTEOMICS   |   CENTER   |   SERVICES   |   EDUCATION    
Overview 
FFE 
MARC 
LC-ESI MS 
PF-2D 
MALDI-TOF 
MALDI-TOF/TOF 
2DE 
Image ºÐ¼® 
2DE package 
DIGE 
Bioinfo Tools 
ÇÁ·ÎÅ×¿È ºÐ¼® Àåºñ 
Related Links 
   HOME > SERVICES > FFE
FFE  
 

4. PF-2D Service

  1. ¼­ºñ½º ¼ö°¡
    - Full service(1D and 2D) : 1,400,000¿ø
    - Only 1-D fractionation: 600,000¿ø

  2. ¼­ºñ½º ÀýÂ÷
    1. ¼Ò¿ä Sample ¾çÀº 3~4mgÀÌ¸ç »óÅ´ °ÇÁ¶»óÅÂÀ̰ųª low salt buffer·Î 200 ul°¡ ³ÑÁö ¾Ê´Â °ÍÀÌ ÁÁ½À´Ï´Ù.
      ( injection Àü¿¡ PF-2D start buffer·Î exchange ÇØ¾ß ÇÔ)
    2. PF-2DÀÇ 1D ´Â ¾à pH 8.5 ¿¡¼­ 4.0 ¿µ¿ª¿¡¼­ ¾à 0.3 unit °£°ÝÀ¸·Î deep 96 plate well·Î ºÐ¸® ¹× ºÐÃë µË´Ï´Ù.
    3. PF-2D ÀÇ 1D Ä÷³À» °ÅÃÄ ³ª¿À´Â fractionÀº ¾à 40°³ Á¤µµ µÇ¸ç fraction volumeÀº 100ul-1000ul·Î ´Ù¾çÇÕ´Ï´Ù.
    4. 1D Ä÷³À» °ÅÃÄ ¹ÞÀº fractionÀº 2D Ä÷³À» °ÉÃÄ ´Ù½Ã 96 plate well·Î fractionation µË´Ï´Ù. 1DÀÇ 1 fraction ´ç ¾à 2°³ÀÇ 96 plate wellÀÌ ¼Ò¿äµÇ¸ç 15ÃÊ °£°ÝÀ¸·Î fractionation ÇÒ °æ¿ì ¾à 150ul¾¿ ¹Þ°Ô µÇ¾î Áý´Ï´Ù.
    5. 1D¿¡¼­ ¹ÞÀº fractionÀº PF 2DÀÇ À̵¿»óÀÌ Æ÷ÇԵǾî ÀÖÀ¸¸ç 2D¸¦ °ÅÄ£ fractionÀÇ °æ¿ì ¹°°ú AcetonitrileÀÌ È¥ÇյǾî ÀÖ½À´Ï´Ù.
    6. ÃÑ ½ÇÇè¿¡ ¼Ò¿äµÇ´Â ½Ã°£Àº ¾à 1ÁÖÀÏÁ¤µµ ÀÔ´Ï´Ù.
    7. °¢ fraction¿¡ ´ëÇÑ Á¤º¸´Â 1D, 2D report¿¡ ³ª¿Í ÀÖ½À´Ï´Ù (retention time, fraction volume, peak areaµî)
    8. Data´Â 1D Å©·Î¸¶Åä±×·¥, 2D °¢ Å©·Î¸¶Åä±×·¥, 1D report, 2D report¸¦ °¢°¢ ÷ºÎÇÏ¿© µå¸³´Ï´Ù.(¾Æ·¡ ÷ºÎ)
    9. ½ÇÇèÀº PF-2DÀÇ 1D¿Í 2D¸¦ ¸ðµÎ ¼­ºñ½º ¹Þ´Â°Í(Full service)¿Í 1-D¸¸ ¼­ºñ½º ¹Þ´Â°ÍÀ¸·Î ³ª´­ ¼öÀÖ½À´Ï´Ù
    10. ½ÇÇè ÀÇ·ÚÀü ¹Ýµå½Ã ´ã´çÀÚ¿Í »óÀÇÇÏ½Ã±æ ¹Ù¶ø´Ï´Ù.





  3. PF-2D ¼­ºñ½º °á°ú ¾ç½Ä :

  4. ¼­ºñ½º ½ÅûÀ» ÇϽñâ Àü, ¹Ýµå½Ã ¿¬±¸¿ø (ÀÌÀº¿µ :02-2123-6625, )°ú ÇØ´ç ¼­ºñ½º¿¡ ´ëÇØ¼­ ²À ¹®ÀÇÇϽŠÈÄ, ¼­ºñ½º ½ÅûÀ» ÇϽñ⠹ٶø´Ï´Ù.

  5. »ùÇà º¸³»½Ç ÁÖ¼Ò : ¼­¿ï ¼­´ë¹®±¸ ½ÅÃ̵¿ ¿¬¼¼´ëÇб³ »êÇÐÇùµ¿°ü 421È£.

  6. ºÐ¼® ¼­ºñ½º ¿ä±ÝÀº ¿¬¼¼´ëÇб³ °èÁ·ΠÀԱݽÃÄÑ ÁÖ½Ã¸é µË´Ï´Ù. ¿¬¼¼´ëÇб³ ¸íÀÇÀÇ °è»ê¼­°¡ ¹ßºÎµÇ¸ç, ¼¼±Ý°è»ê¼­´Â ¹ß±ÞÀÌ ¾ÈµÇ°í, Ä«µå °áÀçµµ ¾ÈµË´Ï´Ù. °áÀç°èÁ¾ȳ»´Â ¸ÞÀÏ·Î µå¸³´Ï´Ù.

2008 © Yonsei Proteome Research Center #422, YPRC/BPRC, Industry-University Research Center, Yonsei Univ., Seodaemun-gu, Seoul, Korea, 120-749
Tel: +82-2-2123-3279, Fax: +82-2-393-6589