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MALDI-TOF  
 

MALDI-TOF (AB 4800 TOF/TOF Analyzer) ¼­ºñ½º ÀýÂ÷ ¹× ¼ö°¡
  1. ¼­ºñ½º ¼ö°¡
    spot from 2-DE or band from 1-D SAS-PAGE
    100,000¿ø
    YPRC¿¡¼­ ºÐ¼®ÇÑ gel¿¡¼­ spotÀ» ¾ò´Â °æ¿ì
    62,000¿ø

  2. ¼­ºñ½º ¼Ò¿ä½Ã°£ : ÃÖ´ë 2ÁÖ (ÀǷڽÿ¡ °á°ú ¼ÛºÎ°¡´ÉÀÏÀ» ¾Ë·Áµå¸³´Ï´Ù)

  3. »ùÇà ÀÇ·Ú: spot À» Àß¶ó ¿À¼Åµµ µÇ°í gelÀ» ±×´ë·Î ÀÇ·ÚÇϼŵµ µË´Ï´Ù
    Àß¶ó¿À½Ã´Â °æ¿ì Àüü gel image ¸¦ °°ÀÌ º¸³»Áֽñ⠹ٶø´Ï´Ù
    data base °Ë»ö¿¡ ÇÊ¿äÇÏ´Ï, ¾î¶² species ÀÎÁö ¾Ë·ÁÁֽñ⠹ٶø´Ï´Ù.

  4. ¼­ºñ½º ÀýÂ÷
    spot from 2-DE -Coomassie staining:
    Spotting- Destaining-trypsin in-gel digestion POROS desalting-MALDI-TOF
    -Database °Ë»ö- monoisotopic peak °ª°ú data base °Ë»ö °á°ú ¼ÛºÎ
    band from 1-DE SDS-PAGE Coomassie staining: spot from 2-DE ¿Í À¯»çÇϳª
    alkylation-reduction °úÁ¤ Ãß°¡µË´Ï´Ù

  5. Database °Ë»öÀº MASCOT, Ms-fit, Profound Áß¿¡ score °¡ ³ôÀº ÂÊÀ¸·Î µå¸³´Ï´Ù.

  6. ¼­ºñ½º ½ÅûÀ» ÇϽñâ Àü, ¹Ýµå½Ã ¿¬±¸¿ø (ÀÌÀº¿µ :02-2123-6625, )°ú ÇØ´ç ¼­ºñ½º¿¡ ´ëÇØ¼­ ²À ¹®ÀÇÇϽŠÈÄ, ¼­ºñ½º ½ÅûÀ» ÇϽñ⠹ٶø´Ï´Ù.

  7. »ùÇà º¸³»½Ç ÁÖ¼Ò : ¼­¿ï ¼­´ë¹®±¸ ½ÅÃ̵¿ ¿¬¼¼´ëÇб³ »êÇÐÇùµ¿°ü 421È£.

  8. ºÐ¼® ¼­ºñ½º ¿ä±ÝÀº ¿¬¼¼´ëÇб³ °èÁ·ΠÀԱݽÃÄÑ ÁÖ½Ã¸é µË´Ï´Ù. ¿¬¼¼´ëÇб³ ¸íÀÇÀÇ °è»ê¼­°¡ ¹ßºÎµÇ¸ç, ¼¼±Ý°è»ê¼­´Â ¹ß±ÞÀÌ ¾ÈµÇ°í, Ä«µå °áÀçµµ ¾ÈµË´Ï´Ù. °áÀç°èÁ¾ȳ»´Â ¸ÞÀÏ·Î µå¸³´Ï´Ù.


  9. ºÐ¼®¼­ºñ½º ¼Ò¿ä½Ã°£
    MALDI-TOF 4 working day (1~30 spots)
    destaining
    2 ½Ã°£ (per 10 spots)
    In-gel digestion
    12 ½Ã°£
    Poros desalting, spotting
    2 ½Ã°£
    MALDI-TOF
    30ºÐ
    Database searching
    2 ½Ã°£

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