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MALDI-TOF (AB 4800 TOF/TOF Analyzer) ¼ºñ½º ÀýÂ÷ ¹× ¼ö°¡
- ¼ºñ½º ¼ö°¡
| spot from 2-DE or band from 1-D SAS-PAGE |
100,000¿ø |
| YPRC¿¡¼ ºÐ¼®ÇÑ gel¿¡¼ spotÀ» ¾ò´Â °æ¿ì |
62,000¿ø |
- ¼ºñ½º ¼Ò¿ä½Ã°£ : ÃÖ´ë 2ÁÖ (ÀǷڽÿ¡ °á°ú ¼ÛºÎ°¡´ÉÀÏÀ» ¾Ë·Áµå¸³´Ï´Ù)
- »ùÇà ÀÇ·Ú: spot À» Àß¶ó ¿À¼Åµµ µÇ°í gelÀ» ±×´ë·Î ÀÇ·ÚÇϼŵµ µË´Ï´Ù
Àß¶ó¿À½Ã´Â °æ¿ì Àüü gel image ¸¦ °°ÀÌ º¸³»Áֽñ⠹ٶø´Ï´Ù
data base °Ë»ö¿¡ ÇÊ¿äÇÏ´Ï, ¾î¶² species ÀÎÁö ¾Ë·ÁÁֽñ⠹ٶø´Ï´Ù.
- ¼ºñ½º ÀýÂ÷
spot from 2-DE -Coomassie staining:
Spotting- Destaining-trypsin in-gel digestion POROS desalting-MALDI-TOF
-Database °Ë»ö- monoisotopic peak °ª°ú data base °Ë»ö °á°ú ¼ÛºÎ
band from 1-DE SDS-PAGE Coomassie staining: spot from 2-DE ¿Í À¯»çÇϳª
alkylation-reduction °úÁ¤ Ãß°¡µË´Ï´Ù
- Database °Ë»öÀº MASCOT, Ms-fit, Profound Áß¿¡ score °¡ ³ôÀº ÂÊÀ¸·Î µå¸³´Ï´Ù.
- ¼ºñ½º ½ÅûÀ» ÇϽñâ Àü, ¹Ýµå½Ã ¿¬±¸¿ø (ÀÌÀº¿µ :02-2123-6625,
)°ú ÇØ´ç ¼ºñ½º¿¡ ´ëÇØ¼ ²À ¹®ÀÇÇϽŠÈÄ, ¼ºñ½º ½ÅûÀ» ÇϽñ⠹ٶø´Ï´Ù.
- »ùÇà º¸³»½Ç ÁÖ¼Ò : ¼¿ï ¼´ë¹®±¸ ½ÅÃ̵¿ ¿¬¼¼´ëÇб³ »êÇÐÇùµ¿°ü 421È£.
- ºÐ¼® ¼ºñ½º ¿ä±ÝÀº ¿¬¼¼´ëÇб³ °èÁ·ΠÀԱݽÃÄÑ ÁÖ½Ã¸é µË´Ï´Ù. ¿¬¼¼´ëÇб³ ¸íÀÇÀÇ °è»ê¼°¡ ¹ßºÎµÇ¸ç, ¼¼±Ý°è»ê¼´Â ¹ß±ÞÀÌ ¾ÈµÇ°í, Ä«µå °áÀçµµ ¾ÈµË´Ï´Ù. °áÀç°èÁ¾ȳ»´Â ¸ÞÀÏ·Î µå¸³´Ï´Ù.
- ºÐ¼®¼ºñ½º ¼Ò¿ä½Ã°£
| MALDI-TOF 4 working day (1~30 spots) |
| destaining |
2 ½Ã°£ (per 10 spots) |
|
| In-gel digestion |
12 ½Ã°£ |
|
| Poros desalting, spotting |
2 ½Ã°£ |
|
| MALDI-TOF |
30ºÐ |
|
| Database searching |
2 ½Ã°£ |
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